Cell Culture Laboratory

Imagen equipment by default
Kind of infrastructure
Platforms/Labs
Typology
Imaging/microscopy
University
Torino (UNITO)
Application / Industrial sector
Agrifood
Health
Location
Department
Department of Agricultural, Forest and Food Sciences
Features
Equipment list
LAB-0190 Inverted optical microscope for in vivo cell cultures with camera for image acquisition
- Visiscope series 400 and camera for image acquisition (video and photos)
connected to a computer

LAB-0191 Class II laminar hood
Nüve MN120 class II laminar hood with sterilization system with UV lamp (220V, 50H)

LAB-0192 Thermostated bath
Nüve bath NB9

LAB-0193 CO2 incubator
170L New Brunswick (Eppendorf) incubator model Galaxy 170 S

LAB-0194 IPP260 Peltier-effect refrigerated incubator
Memmert for the maintenance of insect cell cultures
Volume 256 L, Temperature range 0-70 ° C, double door

LAB-0195 Continuous flow air purifier "GERMREDUC"
- Wall mounted
- UV rays (lambda = 254 nm), usable in the presence of personnel
- Degree of air purification: 92%
- 220 V, 50 Hz
- Lamp life: 6000 hours 1 suction module with 2 UV lamps
- Flow rate: 128 m3 / h

LAB-0196 Leica Greenough stereo microscope
- Zoom factor 6.3: 1 magnification 6.3-80X configuration for reflected light
- Photo / video output

LAB-0197 Fridge / Freezer

LAB-0198 Mitsubishi Air Conditioning
MONO SPLIT system with INVERTER heat pump technology
compliant with ErP regulations with ecological gas R410A for the production of conditioning-heating-dehumidification and air purification
Available measurements
APPLICATIONS AND SERVICES
- Cultivation of human intestinal cells of tumor and non-tumor origin
- Construction of human intestinal models on a three-dimensional level with different types of epithelial cells or PBMCs
- Interaction assays between microorganisms and human intestinal models
- Immunoassays (ELISA), molecular (RT-qPCR, RNA-seq), proliferative and physiological applied to human cells in co-culture
- Analysis of host / human intestinal model interactions through the Trans-epithelial
- Electrical Resistance (TEER)
- Primary cultures of insect immunocytes
- Analysis of the insect's immune system and interactions insect / symbiont and non-symbiotic microorganisms
- Consultancy in experimental design (study models of host interaction human / microorganism, insect / microorganisms)
- Training of new users
- Technical assistance in the interpretation of data
Price list
Rates are defined according to specific requests.
Possibility of on-site measurements
It is possible to access the infrastructure by reservation, accompanied by authorized staff, and / or send samples for analysis (without directly accessing the structure). The technical feasibility of the request is evaluated and the experiments.
Other relevant information
Other contacts:
Rose Marie tedeschi: rosemarie.tedeschi@unito.it
Contact
Person name
Luca Simone COCOLIN
Email
lucasimone.cocolin@unito.it