Leica EM AFS2 (Automated freeze substitution system)

foto_afs
Kind of infrastructure
Singular equipments
Typology
Imaging/microscopy
University
Zaragoza (UNIZAR)
Hubs
Global health
Application / Industrial sector
Circular & Bioeconomy
Health
Description of equipment

Automated freeze substitution and low-temperature embedding system for cryo-fixed biological samples. It enables controlled substitution of vitrified water with organic solvents, followed by resin infiltration and polymerization under precisely regulated low-temperature conditions, ensuring high reproducibility and optimal preservation of ultrastructure for electron microscopy.

The system is particularly suited for downstream processing of samples prepared by high-pressure freezing.

Location
Service
Biological Systems Electron Microscopy Service
Features
Technicians
Yes
Equipment list
Leica EM AFS2 (Automated freeze substitution system)
Sample preparation
TO BE DISCUSSED WITH TECHNICIANS
Price list
https://sai.unizar.es/microscopia-electronica-de-sistemas-biologicos/tarifas
Other relevant information
Services offered:

• Freeze substitution of cryo-fixed samples.
• Low-temperature resin embedding.

Typical research applications:

• Cell Biology.
• Structural Biology.
• Biomedicine.
• Microbiology.
• Biomaterials Science.
• Biotechnology.
Contact
Person name
María José Marín
Email
smesb@unizar.es
Phone
(34) 976 76 16 85