Imaging Platform

Imaging Platform
Kind of infrastructure
Platforms/Labs
Typology
Imaging/microscopy
University
Brescia
Hubs
Global health
Application / Industrial sector
Agrifood
Description of equipment

Zeiss LSM900 Confocal Microscope

An inverted confocal microscope with a motorized xy and z stage, equipped with four solid-state visible lasers (405–488–561–640 nm) and two spectral photomultiplier detectors. 

Integrated Platform for Confocal, Two-Photon, Super-Resolution and Fluorescence Lifetime Imaging (FLIM): Zeiss LSM880. Upright Microscope

An upright microscope with a motorized xy and z stage equipped with two visible lasers (488 and 543 nm) and one IR laser, enabling confocal microscopy, two-photon microscopy, or fluorescence lifetime imaging (FLIM, PicoQuant®) on ex vivo samples. Inverted Microscope  with a motorized xy and z stage equipped with: Three visible lasers (488, 543, 633 nm) and One IR laser. All microscope are complemented with Airyscan super-resolution system, capable of operating in fast mode and a fluorescence lifetime imaging microscopy (FLIM) systemes .

Image Analysis Workstation: a dedicated image analysis station enabling processing and analysis of acquisitions performed using: Zen Black and Zen Blue software (Zeiss) and FLIM software (PicoQuant®).

The X-CLARITY System (Logos Biosystems) is optimized for degassing, embedding, and clearing thick biological samples.

Lightsheet MIcroscopy 

The IVIS Lumina III (PerkinElmer) is an in vivo imaging system that enables the detection and quantification of bioluminescence (BLI) and fluorescence signals in small rodents.

The MSOT system (iThera Medical) is a photoacoustic imaging instrument that enables detection of specific signals within small rodents using low-frequency ultrasound, with a resolution of approximately 150 µm.

Axioobserver for time lapse videomiscoscopy

Location
Institute
University of Brescia (UNIBS), Viale Europa, 11 Brescia
Department
Molecular and Translational Medicine (DMMT)
Features
Prerequisites
Samples should be optically clear
Sample preparation
Yes
Price list
To be defined
Possibility of on-site measurements
Yes
Contact
Person name
Prof.ssa Stefania Mitola, Prof.ssa Arianna Bellucci
Email
stefania.mitola@unibs.it
Phone
+390303717312