Kind of infrastructure
Services
Typology
Biology / genomics / proteomics
University
Zaragoza (UNIZAR)
Application / Industrial sector
Health
Description of equipment
Molecular characterization of microorganisms based on Pulsed-field Gel Electrophoresis (PFGE)
Location
Institute
Agri-Food Research Institute of Aragon IA2
Department
Food Hygiene. Animal Production and Food Science department.
Service
Pulsed-field Gel Electrophoresis (PFGE)
Features
Prerequisites
None
Technicians
The service counts with specialists to develop the analyses.
Equipment list
CHEF-DR® III System (BIORAD) / DCODETM
Available measurements
1. Preparation of the plugs including the microorganisms to identify
2. Pulsed-field Gel Electrophoresis (PFGE)
3. Agarose gel preparation (Gold Agarose -SeaKem®)
4. Gel staining (Gel Red)
5. Results interpretation
2. Pulsed-field Gel Electrophoresis (PFGE)
3. Agarose gel preparation (Gold Agarose -SeaKem®)
4. Gel staining (Gel Red)
5. Results interpretation
Sample preparation
There are several possibilities: raw samples/ pure cultures/ already prepared plugs.
Deployment of the results
Results can be delivered as a picture of the gel or complemented by a report.
Approximate time for the results
At least one week from the reception of the samples. The time could be increased depending on the current demand.
Price list
1. Preparation of the plugs including the microorganisms to identify: to estimate
2. Pulsed-field Gel Electrophoresis (PFGE): 23.11€ for 7 samples batch
3. Agarose gel preparation (Gold Agarose -SeaKem®): 43.18€ each gel
4. Gel staining (Gel Red): 25.45€ each gel staining
5. Results interpretation: to estimate
2. Pulsed-field Gel Electrophoresis (PFGE): 23.11€ for 7 samples batch
3. Agarose gel preparation (Gold Agarose -SeaKem®): 43.18€ each gel
4. Gel staining (Gel Red): 25.45€ each gel staining
5. Results interpretation: to estimate
Possibility of on-site measurements
Measurements will be done by the specialists of the service.
Other relevant information
Email contacts: crota@unizar.es / conchell@unizar.es / mjserran@unizar.es
Address: Veterinary Faculty. Miguel Servet st 177, Zaragoza, Spain. Zip Code: 50013
Address: Veterinary Faculty. Miguel Servet st 177, Zaragoza, Spain. Zip Code: 50013
Contact
Person name
Carmina Rota / Pilar Conchello / María Jesús Serrano
Email
crota@unizar.es
Phone
0034876554144 / 0034876554144 / 0034976762830