Cryostat is a specialized instrument composed of a tissue microtome (cryotome) installed within a cooling chamber, that allows for rapid freezing of tissues and a continuous process of cutting tissue at low cryogenic temperatures (typically around −15 to −30 °C). Low temperatures may be maintained within a cryostat by automated electrical refrigeration systems with accurate thermostats. The selected portion of tissue to freeze is placed on a prepared metal mounting base called chuck, and the tissue encased in a squirt of rapidly freezing material that is liquid at room temperature, but quickly solidifies into a white waxy material in the cryostat. The chuck is then screwed into the blade/plate cutting machinery and the blade gradually brings up to the face of the frozen tissue block where sections can be cut and placed on microscope slides for future analysis