OPEN LAB DSV - Droplet Digital PCR MiSeq / Bio-plex 200

Imagen equipment by default
Kind of infrastructure
Platforms/Labs
Typology
Biology / genomics / proteomics
University
Torino (UNITO)
Application / Industrial sector
Agrifood
Circular & Bioeconomy
Health
Smart technologies
Description of equipment

APPLICATIONS AND SERVICES 
 
Applications 
1)Droplet Digital PCR: 
- detection and monitoring of antibiotic resistance 
- quantification of residual DNA and libraries 
- detection and verification of Genome Editing 
- Copy Number Variation (CNV) analysis, gene expression, microRNA 
- identification of rare mutations and alleles 
 
2) MiSeq Illumina: 
- microbiome analysis and microbial biodiversity (Metagenomics 16S) 
- re-sequencing of genes of interest 
- genetic screening 
- forensic sequencing 
- small genome sequencing, RNA and HLA 
 
3)Bio-Plex 200: 
- molecular pathway analysis 
- development of multiplexing tests for proteins and nucleic acids 
- immunoassays and antibody tests 
 
Services 
- Experimental design consultancy and technical assistance; for NGS experiments 
- consulting in data analysis

Location
Department
Department of Veterinary Sciences
Features
Equipment list
LAB-0133 Platform for Droplet Digital PCR (Bio-Rad)
QX-200 Droplet Generator
PX1 PCR Plate Sealer
C1000 Touch Thermal Cycler
QX-200 Droplet Reader

LAB-0134 Illumina MiSeq for next-generation sequencing
- Flexible running parameters, in terms of sequence length
- Mode of reading fragments (single or paired-end)
- System performance: 540Mbp to 15Gbp depending on chemistry and parameters used

LAB-0135 Bio-plex 200 platform Luminex technology (Bio-Rad)
Bio-Plex 200 System array reader: allows you to identify and quantify up to 100 different analytes (proteins and nucleic acids) simultaneously, supports plates 96-well multiwell; Bio-Plex Pro Wash Station: For automated washing, supports 96 multiwell plates wells.
Price list
Rates are defined according to specific requests.
Other relevant information
correlated web pages:
- https://veteren.campusnet.unito.it/do/home.pl/View?doc=/research/infrastructures/open_lab/bio-plex_luminex.html
- https://veteren.campusnet.unito.it/do/home.pl/View?doc=/research/infrastructures/open_lab/miseq.html
Contact
Person name
Annalisa PETRELLI
Email
openlab.dsv@unito.it